Recent advances in myeloid-derived suppressor cell biology

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Entosis: From Cell Biology to Clinical Cancer Pathology

Entosis is a phenomenon, in which one cell enters a second one. New clinico-histopathological studies of entosis prompted us to summarize its significance in cancer. It appears that entosis might be a novel, independent prognostic predictor factor in cancer histopathology.

We briefly discuss the biological basis of entosis, followed by a summary of published clinico-histopathological studies on entosis significance in cancer prognosis. The correlation of entosis with cancer prognosis in head and neck squamous cell carcinoma, anal carcinoma, lung adenocarcinoma, pancreatic ductal carcinoma and breast ductal carcinoma, is shown.

Numerous entotic figures are associated with a more malignant cancer phenotype and poor prognosis in many cancers. We also showed that some anticancer drugs could induce entosis in cell culture, even as an escape mechanism. Thus, entosis is likely beneficial for survival of malignant cells, i.e., an entotic cell can hide from unfavourable factors in another cell and subsequently leave the host cell remaining intact, leading to failure in therapy or cancer recurrence.

Finally, we highlight the potential relationship of cell adhesion with entosis in vitro, based on the model of the BxPc3 cells cultured in full adhesive conditions, comparing them to a commonly used MCF7 semiadhesive model of entosis.

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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

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Description: Our Ras Activation Assays use visible agarose beads to selectively precipitate the active form of specific Ras protein of interest. The precipitated small GTPase is then detected by Western blot using a target-specific antibody included in the kit. Assays are available to detect specific isoforms H-Ras, K-Ras, and N-Ras, as well as a Pan-Ras assay that detects all three isoforms.

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STA-402 20 assays
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rac1 Activation Assay Kit, Trial Size

STA-401-1-T 5 assays
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Description: Our Rac Activation Assays use visible agarose beads to selectively precipitate the active form of Rac1 or Rac2. The precipitated small GTPase is then detected by Western blot using a Rac1- or Rac2-specific antibody included in the kit.

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STA-402-T 5 assays
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Description: Our Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of Cdc42 protein. The precipitated small GTPase is then detected by Western blot using a Cdc42-specific antibody included in the kit.

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STA-403-A-T 5 assays
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

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STA-403-B-T 5 assays
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

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STA-403-C-T 5 assays
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Description: Our Rho Activation Assays use visible agarose beads to selectively precipitate the active form of RhoA, RhoB or RhoC. The precipitated small GTPase is then detected by Western blot using a RhoA-, RhoB- or RhoC-specific antibody included in the kit.

Rac1/Cdc42 Activation Assay Combo Kit

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Description: Our Rac1/Cdc42 Activation Assays use visible agarose beads to selectively precipitate the active form of the small GTPase. The precipitated small GTPase is then detected by Western blot using a specific antibody included in the kit.

Rap1 Activation Assay Kit, Trial Size

STA-406-1-T 5 assays
EUR 403
Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Rap2 Activation Assay Kit, Trial Size

STA-406-2-T 5 assays
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Description: Our Rap Activation Assays use visible agarose beads to selectively precipitate the active form of Rap1 or Rap2. The precipitated small GTPase is then detected by Western blot using a Rap1- or Rap2-specific antibody included in the kit.

Arf1 Activation Assay Kit, Trial Size

STA-407-1-T 5 assays
EUR 403
Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

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Description: Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

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STA-408-T 5 assays
EUR 403
Description: Our Ral Activation Assay uses visible agarose beads to selectively precipitate the active form of Ral protein. The precipitated small GTPase is then detected by Western blot using a Ral-specific antibody included in the kit.

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STA-409-T 5 assays
EUR 403
Description: Our Ran Activation Assay uses visible agarose beads to selectively precipitate the active form of Ran protein. The precipitated small GTPase is then detected by Western blot using a Ran-specific antibody included in the kit.

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Recent advances in myeloid-derived suppressor cell biology

In recent years, studying the role of myeloid-derived suppressor cells (MDSCs) in many pathological inflammatory conditions has become a very active research area. Although the role of MDSCs in cancer is relatively well established, their role in non-cancerous pathological conditions remains in its infancy resulting in much confusion.

Our objectives in this review are to address some recent advances in MDSC research in order to minimize such confusion and to provide an insight into their function in the context of other diseases. The following topics will be specifically focused upon: (1) definition and characterization of MDSCs; (2) whether all MDSC populations consist of immature cells;

 (3) technical issues in MDSC isolation, estimation and characterization; (4) the origin of MDSCs and their anatomical distribution in health and disease; (5) mediators of MDSC expansion and accumulation; (6) factors that determine the expansion of one MDSC population over the other; (7) the Yin and Yang roles of MDSCs. Moreover, the functions of MDSCs will be addressed throughout the text.

MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis?

The discovery of MRGPRX2 marks an important change in MC biology, explaining non-IgE-mediated clinical phenomena relying on MCs. As receptor for multiple drugs, MRGPRX2 is crucial to drug-induced hypersensitivity.

 However, not only drugs, but also endogenous mediators like neuropeptides and host defense peptides activate MRGPRX2, suggesting its broad impact in cutaneous pathophysiology. Here, we give a brief overview of MRGPRX2 and its regulation by microenvironmental stimuli, which support MCs and can be altered in skin disorders, and briefly touch on the functional programs elicited by MRGPRX2 ligation. Studies in Mrgprb2-deficient mice (the murine ortholog) help illuminate MRGPRX2’s function in health and disease.

Recent advances in this model support the long-suspected operational unit between MCs and nerves, with MRGPRX2 being a vital component. Based on the limited evidence for a major contribution of FcεRI/IgE-activated MCs to atopic dermatitis (AD), we develop the hypothesis that MRGPRX2 constitutes the missing link connecting MCs and AD, at least in selected endotypes. Support comes from the multifold changes in the MC-neuronal system of AD skin (e.g. greater density of MCs and closer connections between MCs and nerves, increased PAR-2/Substance P).

 We theorize that these deregulations suffice to initiate AD, but external triggers, many of which activating MRGPRX2 themselves (e.g. Staphylococcus aureus) further feed into the loop. Itch, the most burdensome hallmark of AD, is mostly non-histaminergic but tryptase-dependent, and tryptase is preferentially released upon MRGPRX2 activation. Because MRGPRX2 is a very active research field, some of the existing gaps are likely to be closed soon.

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

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anti- 6*His, His-Tag antibody

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anti- 6*His, His-Tag antibody

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anti- 6*his,His tag antibody

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Description: Antibody raised against 6*his,His tag

6X His tag monoclonal antibody

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Description: 6X His tag monoclonal antibody secondary antibody

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TSH (Thyrotrophin) ELISA test

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80R-4076 20 ug
EUR 327
Description: Recombinant Human MAP2K6 protein (His tag)

THOC7 protein (His tag)

80R-4077 50 ug
EUR 435
Description: Recombinant Human THOC7 protein (His tag)

STOML1 protein (His tag)

80R-4078 50 ug
EUR 327
Description: Recombinant Human STOML1 protein (His tag)

IL12RB1 protein (His tag)

80R-4079 50 ug
EUR 435
Description: Recombinant Human IL12RB1 protein (His tag)

CLEC5A protein (His tag)

80R-4080 50 ug
EUR 327
Description: Recombinant Human CLEC5A protein (His tag)

ATP6V1F protein (His tag)

80R-4081 100 ug
EUR 327
Description: Recombinant Human ATP6V1F protein (His tag)

NRF2 protein (His tag)

80R-4082 20 ug
EUR 327
Description: Recombinant Human NRF2 protein (His tag)

KLK13 protein (His tag)

80R-4083 10 ug
EUR 327
Description: Recombinant Human KLK13 protein (His tag)

TSLP protein (His tag)

80R-4084 20 ug
EUR 327
Description: Recombinant Human TSLP protein (His tag)

IL34 protein (His tag)

80R-4085 50 ug
EUR 327
Description: Recombinant Mouse IL34 protein (His tag)

STK11 protein (His tag)

80R-4086 50 ug
EUR 327
Description: Recombinant Human STK11 protein (His tag)

FGG protein (His tag)

80R-4087 20 ug
EUR 327
Description: Recombinant Human FGG protein (His tag)

EDN3 protein (His tag)

80R-4088 10 ug
EUR 327
Description: Recombinant Human EDN3 protein (His tag)

IFNL1 protein (His tag)

80R-4089 100 ug
EUR 327
Description: Recombinant Human IFNL1 protein (His tag)

ACPP protein (His tag)

80R-4090 100 ug
EUR 435
Description: Recombinant Human ACPP protein (His tag)

AZGP1 protein (His tag)

80R-4091 100 ug
EUR 435
Description: Recombinant Human AZGP1 protein (His tag)

PRMT3 protein (His tag)

80R-4092 100 ug
EUR 327
Description: Recombinant Human PRMT3 protein (His tag)

ACP2 protein (His tag)

80R-4093 100 ug
EUR 327
Description: Recombinant Human ACP2 protein (His tag)

PRSS7 protein (His tag)

80R-4094 100 ug
EUR 327
Description: Recombinant Human PRSS7 protein (His tag)

FARSB protein (His tag)

80R-4095 100 ug
EUR 327
Description: Recombinant Human FARSB protein (His tag)

APMAP protein (His tag)

80R-4096 100 ug
EUR 327
Description: Recombinant Human APMAP protein (His tag)

tnaa protein (His tag)

80R-4097 100 ug
EUR 327
Description: Recombinant Human tnaa protein (His tag)

FST protein (His tag)

80R-4098 50 ug
EUR 327
Description: Recombinant Human FST protein (His tag)

IL9 protein (His tag)

80R-4099 50 ug
EUR 327
Description: Recombinant Human IL9 protein (His tag)

SFRP5 protein (His tag)

80R-4100 100 ug
EUR 327
Description: Recombinant Human SFRP5 protein (His tag)

CYTIP protein (His tag)

80R-4101 100 ug
EUR 327
Description: Recombinant Human CYTIP protein (His tag)

FOSL2 protein (His tag)

80R-4102 50 ug
EUR 327
Description: Recombinant Human FOSL2 protein (His tag)

SPI1 protein (His tag)

80R-4103 50 ug
EUR 327
Description: Recombinant Human SPI1 protein (His tag)

CD9 protein (His tag)

80R-4104 100 ug
EUR 435
Description: Recombinant Human CD9 protein (His tag)

LRP2BP protein (His tag)

80R-4105 100 ug
EUR 327
Description: Recombinant Human LRP2BP protein (His tag)

MTHFD2 protein (His tag)

80R-4106 100 ug
EUR 327
Description: Recombinant Human MTHFD2 protein (His tag)

GORASP2 protein (His tag)

80R-4107 100 ug
EUR 327
Description: Recombinant Human GORASP2 protein (His tag)

RASD2 protein (His tag)

80R-4108 100 ug
EUR 327
Description: Recombinant Human RASD2 protein (His tag)

IP6K2 protein (His tag)

80R-4109 50 ug
EUR 435
Description: Recombinant Human IP6K2 protein (His tag)

ANGPTL2 protein (His tag)

80R-4110 50 ug
EUR 435
Description: Recombinant Human ANGPTL2 protein (His tag)

AGA protein (His tag)

80R-4111 100 ug
EUR 327
Description: Recombinant Human AGA protein (His tag)

GZMB protein (His tag)

80R-4112 100 ug
EUR 327
Description: Recombinant Human GZMB protein (His tag)

INHBA protein (His tag)

80R-4113 100 ug
EUR 327
Description: Recombinant Human INHBA protein (His tag)

PEPD protein (His tag)

80R-4114 100 ug
EUR 327
Description: Recombinant Human PEPD protein (His tag)

ppiA protein (His tag)

80R-4115 100 ug
EUR 327
Description: Recombinant Human ppiA protein (His tag)

Akr1b1 protein (His tag)

80R-4116 100 ug
EUR 327
Description: Recombinant Human Akr1b1 protein (His tag)

ppiA protein (His tag)

80R-4117 100 ug
EUR 327
Description: Recombinant Human ppiA protein (His tag)

TFRC protein (His tag)

80R-4118 100 ug
EUR 327
Description: Recombinant Human TFRC protein (His tag)

PRKAA1 protein (His tag)

80R-4119 100 ug
EUR 327
Description: Recombinant Human PRKAA1 protein (His tag)

IMPAD1 protein (His tag)

80R-4120 10 ug
EUR 327
Description: Recombinant Human IMPAD1 protein (His tag)

Gapth protein (His tag)

80R-4122 100 ug
EUR 327
Description: Recombinant Human Gapth protein (His tag)

CTSL protein (His tag)

80R-4123 100 ug
EUR 327
Description: Recombinant Human CTSL protein (His tag)

REG4 protein (His tag)

80R-4124 100 ug
EUR 327
Description: Recombinant Human REG4 protein (His tag)

IGFBP3 protein (His tag)

80R-4125 100 ug
EUR 327
Description: Recombinant Human IGFBP3 protein (His tag)

PPM1D protein (His tag)

80R-4126 50 ug
EUR 327
Description: Recombinant Human PPM1D protein (His tag)

CYP2E1 protein (His tag)

80R-4127 100 ug
EUR 327
Description: Recombinant Human CYP2E1 protein (His tag)

CTGF protein (His tag)

80R-4128 100 ug
EUR 327
Description: Recombinant Human CTGF protein (His tag)

Anxa1 protein (His tag)

80R-4129 100 ug
EUR 327
Description: Recombinant Human Anxa1 protein (His tag)

PCOLCE protein (His tag)

80R-4130 100 ug
EUR 327
Description: Recombinant Human PCOLCE protein (His tag)

CCL25 protein (His tag)

80R-4131 100 ug
EUR 327
Description: Recombinant Human CCL25 protein (His tag)

CD70 protein (His tag)

80R-4132 100 ug
EUR 327
Description: Recombinant Human CD70 protein (His tag)

JMJD6 protein (His tag)

80R-4133 50 ug
EUR 435
Description: Recombinant Human JMJD6 protein (His tag)

BDNF protein (His tag)

80R-4134 100 ug
EUR 327
Description: Recombinant Human BDNF protein (His tag)

GNG12 protein (His tag)

80R-4135 100 ug
EUR 327
Description: Recombinant Human GNG12 protein (His tag)

CTHRC1 protein (His tag)

80R-4136 100 ug
EUR 327
Description: Recombinant Human CTHRC1 protein (His tag)

TGM2 protein (His tag)

80R-4137 50 ug
EUR 435
Description: Recombinant Human TGM2 protein (His tag)

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