Biomaterial-Driven Immunomodulation: CellBiology-Based Strategies to Mitigate Severe Inflammation and Sepsis
Inflammation is an essential component of a wide variety of disease processes and oftentimes can increase the deleterious effects of a disease. Finding ways to modulate this essential immune process is the basis for many therapeutics under development and is a burgeoning area of research for both basic and translational immunology.
In addition to developing therapeutics for cellular and molecular targets, the use of biomaterials to modify innate and adaptive immune responses is an area that has recently sparked significant interest. In particular, immunomodulatory activity can be engineered into biomaterials to elicit heightened or dampened immune responses for use in vaccines, immune tolerance, or anti-inflammatory applications.
Importantly, the inherent physicochemical properties of the biomaterials play a significant role in determining the observed effects. Properties including composition, molecular weight, size, surface charge, and others affect interactions with immune cells (i.e., nano-bio interactions) and allow for differential biological responses such as activation or inhibition of inflammatory signaling pathways, surface molecule expression, and antigen presentation to be encoded.
Numerous opportunities to open new avenues of research to understand the ways in which immune cells interact with and integrate information from their environment may provide critical solutions needed to treat a variety of disorders and diseases where immune dysregulation is a key inciting event. However, to elicit predictable immune responses there is a great need for a thorough understanding of how the biomaterial properties can be tuned to harness a designed immunological outcome.
This review aims to systematically describe the biological effects of nanoparticle properties-separate from additional small molecule or biologic delivery-on modulating innate immune cell responses in the context of severe inflammation and sepsis. We propose that nanoparticles represent a potential polypharmacological strategy to simultaneously modify multiple aspects of dysregulated immune responses where single target therapies have fallen short for these applications.
This review intends to serve as a resource for immunology labs and other associated fields that would like to apply the growing field of rationally designed biomaterials into their work.
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.
Description: ELISA based test for quantitative detection of HCG?Anti-Human Chorionic Gonadotrophin Antibody IgG)
4D CellBiology: Adaptive optics lattice light-sheet imaging and AI powered big data processing of live stem cell-derived organoids
New methods in stem cell 3D organoid tissue culture, advanced imaging, and big data image analytics now allow tissue-scale 4D cell biology but currently available analytical pipelines are inadequate for handing and analyzing the resulting gigabytes and terabytes of high-content imaging data. We expressed fluorescent protein fusions of clathrin and dynamin2 at endogenous levels in genome- edited human embryonic stem cells, which were differentiated into intestinal epithelial organoids.
Lattice light-sheet imaging with adaptive optics (AO-LLSM) allowed us to image large volumes of these organoids (70 × 60 × 40 μm xyz) at 5.7 s/frame. We developed an open-source data analysis package termed pyLattice to process the resulting large (∼60 Gb) movie data sets and to track clathrin-mediated endocytosis (CME) events.
We then expressed fluorescent protein fusions of actin and tubulin in genome-edited induced human pluripotent stem cells, which were differentiated into human cortical organoids. Using the AO-LLSM mode on the new MOSAIC (Multimodal Optical Scope with Adaptive Imaging Correction) allowed us to image neuronal migration deep in the organoid. We augmented pyLattice with a deep learning module and used it to process the brain organoid data.
Common Sources of Inflammation and Their Impact on Hematopoietic Stem CellBiology
Purpose of review: Inflammatory signals have emerged as critical regulators of hematopoietic stem cell (HSC) function. Specifically, HSCs are highly responsive to acute changes in systemic inflammation and this influences not only their division rate but also their lineage fate. Identifying how inflammation regulates HSCs and shapes the blood system is crucial to understanding the mechanisms underpinning these processes, as well as potential links between them.
Recent findings: A widening array of physiologic and pathologic processes involving heightened inflammation are now recognized to critically affect HSC biology and blood lineage production. Conditions documented to affect HSC function include not only acute and chronic infections but also autoinflammatory conditions, irradiation injury, and physiologic states such as aging and obesity.
Summary: Recognizing the contexts during which inflammation affects primitive hematopoiesis is essential to improving our understanding of HSC biology and informing new therapeutic interventions against maladaptive hematopoiesis that occurs during inflammatory diseases, infections, and cancer-related disorders.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: This cell lysate is prepared from human mcf-7 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein from Human Tumor Cell Line: MCF 7
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.
Paraffin Tissue Section - Human Tumor Cell Line: MCF-7
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.
Description: pre-made lentiviral particles expressing β-Lactamase gene. A firefly luciferase marker was expressed under RSV promoter. Particles is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
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 IgM Rapid Test Kit)
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit)
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 IgG Rapid Test Kit)
 IgM Rapid Test Kit)
 IgG Rapid Test Kit)
 IgM Rapid Test Kit)
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 IgG Rapid Test Kit)
 IgM Rapid Test Kit)
 IgM / IgG Rapid Test Kit)
 Antigen Rapid Test Kit)
 IgG Rapid Test Kit)
 IgG Rapid Test Kit)
 Antibody Rapid Test Kit)
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, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site))
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SKOV-3/Luc Cell Line 
MCF-7 cells 
cDNA from Human Tumor Cell Line: MCF 7 
Human Mcf-7 Whole Cell Lysate 
MCF-7 Nuclear Extract 
Genomic DNA from Human Tumor Cell Line: MCF 7 
Total Protein from Human Tumor Cell Line: MCF 7 
Membrane Protein from Human Tumor Cell Line: MCF 7 
Total RNA from Human Tumor Cell Line: MCF 7 
Paraffin Tissue Section - Human Tumor Cell Line: MCF-7 )
MCF-7 Nuclear Extract (H2O2) 
Human AsPC1 / Luciferase Cell Line 
mouse MOPC315 / Luciferase Cell Line  Cell Nuclear Extract)
Human MCF-7 (breast cancer) Cell Nuclear Extract  stable cell line)
A549 / Luciferase (Puromycin) stable cell line  Cell Line)
Human PANC-1 / Luciferase (Puro) Cell Line )
MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells) 
MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes 
AAV2-Luc Control Virus 
AAV1-Luc Control Virus 
AAV3-Luc Control Virus 
AAV4-Luc Control Virus 
AAV5-Luc Control Virus 
AAV6-Luc Control Virus 
MCF-10A  Whole Cell Lysate, Hydrogen Peroxide Stimulated)
Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated 
293AD Cell Line 
293AAV Cell Line 
293LTV Cell Line 
293RTV Cell Line 
293/GFP Cell Line 
T47D/GFP Cell Line 
A549/GFP Cell Line 
HeLa/GFP Cell Line 
NIH3T3/GFP Cell Line 
NIH3T3/Cas9 Cell Line 
293/Cas9 Cell Line 
HeLa/Cas9 Cell Line 
OVCAR-5/RFP Cell Line 
NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line 
Platinum-E Retroviral Packaging Cell Line, Ecotropic 
Platinum-A Retroviral Packaging Cell Line, Amphotropic 
Platinum-GP Retroviral Packaging Cell Line, Pantropic 
pT7- Luc 
pSBE- Luc 
pTRE3G- LUC 
pAP1- Luc 
pHSE- Luc 
pGRE- luc 
pCRE- Luc 
pViperin- Luc 
pTA- Luc 
pTAL- Luc 
pIRF3- Luc 
p53- Luc 
pBV-Luc 
pMR-Luc 
Total Protein - Murine Embryonic Stem Cell Line D3 
Anti-Cytokeratin 7 
Individual Reaction Mix 7 
Recombinant Human Galectin-7 
Recombinant Human Interleukin-7 
Recombinant Mouse Interleukin-7 
Recombinant Human Kallikrein-7 
Recombinant ProMatrix Metalloproteinase-7  lentiviral particles)
β-Lactamase (Luciferase) lentiviral particles 
Huamn SW403 / Luciferase Stable Cells 
pUC57-Tac-Luc 
p21-Luc Plasmid 
IgK- IFN- luc 
pNFAT- TA- Luc 
pE2F- TA- Luc 
pISRE- TA- Luc 
pGAS- TA- Luc 
pP53- TA- luc 
pStat3- TA- luc - Luc)
pHes1 (2.5k)- Luc - luc)
pHes1 (467)- luc 
pNF-kB-Luc 
pGL3-ELAM-Luc 
proE-cad178-Luc 
pSynSRE-T-Luc 
Recombinant Human Interleukin-7, Saccharomyces 
Recombinant Human Matrix Metalloproteinase-7  ELISA Kit)
Monkey Macrophage chemotactic Factor (MCF) ELISA Kit