Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT

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fmblab

Propofol Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

Background: Propofol is a common sedative-hypnotic drug traditionally used for inducing and maintaining general anesthesia. Recent studies have drawn attention to the nonanesthetic effects of propofol, but the potential mechanism by which propofol suppresses non-small-cell lung cancer (NSCLC) progression has not been fully elucidated.

Methods: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to treat A549 cells for 1, 4, and 12 hours and Cell Counting Kit-8 (CCK-8) to detect proliferation. Apoptosis was measured with flow cytometry. We also transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or negative control ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 small interfering ribonucleic acid (siRNA) or negative control. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression were detected using Western blotting, whereas miR-21 expression was examined by real-time polymerase chain reaction (RT-PCR). In vivo, nude mice were given injections of A549 cells to grow xenograft tumors; 8 days later, the mice were intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors were then collected from mice and analyzed by immunohistochemistry and Western blotting.

Results: Propofol inhibited growth (1 hour, P = .001; 4 hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; 4 hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent manner. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive effects of propofol on A549 cells by decreasing PTEN protein

expression (mean differences [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), resulting in an increase in pAKT levels (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol exposure. In vivo, propofol treatment reduced NSCLC tumor growth (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

Conclusions: Our study indicated that propofol inhibited A549 cell growth, accelerated apoptosis via the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell growth, and promoted apoptosis in vivo. Our findings provide new implications for propofol in cancer therapy and indicate that propofol is extremely advantageous in surgical treatment.

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EF1a control lentivirus (Hygro)
EF1a-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.
EF1a control lentivirus (Zeo)
EF1a-Null-Zeo 1 x107 IFU/ml x 200ul
EUR 349
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the Zeocin selection under RSV promoter.
GFP Expressing Human Glioblastoma Cells
TR01-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Gastric Carcinoma N87 Cells
TR02-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)
TR04-GFP 500,000 Cells
EUR 1354
GFP Expressing Human Prostate Carcinoma Cells (DU 145)
TR03-GFP 500,000 Cells
EUR 1354
CamKII-Luc (GFP) Lentivirus 
LVP1055-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human CamKII's promoter which restrictly expressed in neurons of the neocortex and hippocampus, also pyramidal neurons. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CamKII-Rluc (GFP) Lentivirus 
LVP1056-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human CamKII's promoter which restrictly expressed in neurons of the neocortex and hippocampus, also pyramidal neurons. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-GFP (Bsd) Lentivirus 
LVP1104-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-GFP (Bsd) Lentivirus 
LVP1104-B-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-GFP (Neo) Lentivirus 
LVP1104-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-GFP (Neo) Lentivirus 
LVP1104-N-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-GFP (Puro) Lentivirus 
LVP1104-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-GFP (Puro) Lentivirus 
LVP1104-P-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-GFP (RFP) Lentivirus 
LVP1104-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-GFP (RFP) Lentivirus 
LVP1104-R-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-RFP (GFP) Lentivirus 
LVP1105-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-RFP (GFP) Lentivirus 
LVP1105-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-Luc (GFP) Lentivirus 
LVP1106-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-Luc (GFP) Lentivirus 
LVP1106-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.
Nephrin-Rluc (GFP) Lentivirus 
LVP1107-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Nephrin-Rluc (GFP) Lentivirus 
LVP1107-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
EGR1-GFP (Bsd) Lentivirus 
LVP1119-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-GFP (Bsd) Lentivirus 
LVP1119-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Blasticidin selection marker under RSV promoter, provided in PBS solution.
EGR1-GFP (Neo) Lentivirus 
LVP1119-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-GFP (Neo) Lentivirus 
LVP1119-N-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Neomycin selection marker under RSV promoter, provided in PBS solution.
EGR1-GFP (Puro) Lentivirus 
LVP1119-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the early growth response gene (EGR1). This lentivirus contains the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-GFP (Puro) Lentivirus 
LVP1119-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the early growth response gene (EGR1). This lentivirus contains the puromycin selection marker under RSV promoter, provided in PBS solution.
EGR1-GFP (RFP) Lentivirus 
LVP1119-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker RFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-GFP (RFP) Lentivirus 
LVP1119-R-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker RFP under RSV promoter, provided in PBS solution.
EGR1-RFP (GFP) Lentivirus 
LVP1120-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-RFP (GFP) Lentivirus 
LVP1120-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in PBS solution.
EGR1-Luc (GFP) Lentivirus 
LVP1121-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-Luc (GFP) Lentivirus 
LVP1121-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.
EGR1-Rluc (GFP) Lentivirus 
LVP1122-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-Rluc (GFP) Lentivirus 
LVP1122-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.
GFAP-GFP (Bsd) Lentivirus 
LVP1125-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-GFP (Bsd) Lentivirus 
LVP1125-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-GFP (Neo) Lentivirus 
LVP1125-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-GFP (Neo) Lentivirus 
LVP1125-N-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-GFP (Puro) Lentivirus 
LVP1125-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-GFP (Puro) Lentivirus 
LVP1125-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-GFP (RFP) Lentivirus 
LVP1125-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-GFP (RFP) Lentivirus 
LVP1125-R-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-RFP (GFP) Lentivirus 
LVP1126-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-RFP (GFP) Lentivirus 
LVP1126-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-Luc (GFP) Lentivirus 
LVP1127-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-Luc (GFP) Lentivirus 
LVP1127-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.
GFAP-Rluc (GFP) Lentivirus 
LVP1128-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFAP-Rluc (GFP) Lentivirus 
LVP1128-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.
EBNA1 (GFP-Puro) Lentivirus
LVP1134-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing GFP-Puromycin dual marker.
EBNA2 (GFP-Puro) Lentivirus
LVP1135-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA2 gene under EF1a promoter, containing GFP-Puromycin dual marker.
E1A (GFP-Puro) Lentivirus
LVP1137-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Adenovirus type 5's E1A gene under EF1a promoter, containing GFP-Puromycin dual marker.
HOXA9 (GFP-Puro) Lentivirus
LVP1138-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human HOXA9 gene under EF1a promoter, containing GFP-Puromycin dual marker.
KRas_G12V (GFP-Puro) Lentivirus
LVP1139-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human Kras V12G mutant under EF1a promoter, containing GFP-Puromycin dual marker.
CDK4 (GFP-Puro) Lentivirus
LVP1140-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human CDK4 gene under EF1a promoter, containing GFP-Puromycin dual marker.
cMyc (GFP-Puro) Lentivirus
LVP1141-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human cMyc gene under EF1a promoter, containing GFP-Puromycin dual marker.
AR-GFP (Bsd) Lentivirus
LVP912-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-GFP (Neo) Lentivirus  
LVP912-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-GFP (Puro) Lentivirus
LVP912-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made Lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This Lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-GFP (RFP) Lentivirus 
LVP912-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-RFP (GFP) Lentivirus 
LVP913-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-Luc (GFP) Lentivirus 
LVP914-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-Rluc (GFP) Lentivirus 
LVP915-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-GFP (Bsd) Lentivirus 
LVP937-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-GFP (Neo) Lentivirus 
LVP937-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-GFP (Puro) Lentivirus 
LVP937-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-GFP (RFP) Lentivirus 
LVP937-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-RFP (GFP) Lentivirus 
LVP938-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-Luc (GFP) Lentivirus 
LVP939-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-Rluc (GFP) Lentivirus 
LVP940-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Bsd) Lentivirus 
LVP941-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Neo) Lentivirus 
LVP941-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Puro) Lentivirus 
LVP941-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (RFP) Lentivirus 
LVP941-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-RFP (GFP) Lentivirus 
LVP942-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Luc (GFP) Lentivirus 
LVP943-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Rluc (GFP) Lentivirus 
LVP944-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Bsd) Lentivirus 
LVP945-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Neo) Lentivirus 
LVP945-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Puro) Lentivirus 
LVP945-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (RFP) Lentivirus 
LVP945-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-RFP (GFP) Lentivirus 
LVP946-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Luc (GFP) Lentivirus 
LVP947-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Rluc (GFP) Lentivirus 
LVP948-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Bsd) Lentivirus 
LVP949-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Neo) Lentivirus 
LVP949-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Puro) Lentivirus 
LVP949-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (RFP) Lentivirus 
LVP949-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-RFP (GFP) Lentivirus 
LVP950-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

Establishment of a physiologically relevant human hepatocyte-like cell system for in vitro translational research has been hampered by the limited availability of cell models that accurately reflect human biology and the pathophysiology of human disease. Here we report a robust, reproducible, and scalable protocol for the generation of hepatic organoids from human induced pluripotent stem cells (hiPSCs) using short exposure to nonengineered matrices.

 These hepatic organoids follow defined stages of hepatic development and express higher levels of early (hepatocyte nuclear factor 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this model to explore the biology of the pleiotropic TRIB1 (Tribbles-1) gene associated with a number of metabolic traits, including nonalcoholic fatty liver disease and plasma lipids.

We used genome editing to delete the TRIB1 gene in hiPSCs and compared TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs under both 2D culture and three-dimensional (3D) organoid conditions. Under conventional 2D culture conditions, TRIB1-deficient HLCs showed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In contrast, when cultured as 3D hepatic organoids, the differentiation defects were rescued, and a clear lipid-related phenotype was noted in the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work supports the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, including the functional interrogation of genes identified through human genetic investigation.

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price
Description: The best epigenetics products

MCF 7 Membrane Lysate

XBL-10442 0.1 mg
EUR 516.5
Description: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 207

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 213

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 164

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 128

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 467
  • Category: Exosomes

MCF-10A

C0006015 One Frozen vial
EUR 467

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1354

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 213

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2333

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2333

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2333

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2333

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2333

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2333

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2333

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2333

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1394

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1394

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1394

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1394

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1394

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2333

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2333

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2333

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2333

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2333

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2333

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 712

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Lung Lysate (7 Days Old)

1402-7 0.1 mg
EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)

1403-7 0.1 mg
EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)

1404-7 0.1 mg
EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)

1405-7 0.1 mg
EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)

1406-7 0.1 mg
EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)

1409-7 0.1 mg
EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)

1415-7 0.1 mg
EUR 191
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)

1419-7 0.1 mg
EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)

1420-7 0.1 mg
EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-48T 48T
EUR 402

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-96T 96T
EUR 684

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-48T 48T
EUR 289

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-96T 96T
EUR 466

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