Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT

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fmblab

Propofol Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

Background: Propofol is a common sedative-hypnotic drug traditionally used for inducing and maintaining general anesthesia. Recent studies have drawn attention to the nonanesthetic effects of propofol, but the potential mechanism by which propofol suppresses non-small-cell lung cancer (NSCLC) progression has not been fully elucidated.

Methods: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to treat A549 cells for 1, 4, and 12 hours and Cell Counting Kit-8 (CCK-8) to detect proliferation. Apoptosis was measured with flow cytometry. We also transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or negative control ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 small interfering ribonucleic acid (siRNA) or negative control. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression were detected using Western blotting, whereas miR-21 expression was examined by real-time polymerase chain reaction (RT-PCR). In vivo, nude mice were given injections of A549 cells to grow xenograft tumors; 8 days later, the mice were intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors were then collected from mice and analyzed by immunohistochemistry and Western blotting.

Results: Propofol inhibited growth (1 hour, P = .001; 4 hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; 4 hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent manner. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive effects of propofol on A549 cells by decreasing PTEN protein

expression (mean differences [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), resulting in an increase in pAKT levels (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol exposure. In vivo, propofol treatment reduced NSCLC tumor growth (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

Conclusions: Our study indicated that propofol inhibited A549 cell growth, accelerated apoptosis via the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell growth, and promoted apoptosis in vivo. Our findings provide new implications for propofol in cancer therapy and indicate that propofol is extremely advantageous in surgical treatment.

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CMV control lentivirus (Hygro)
CMV-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 349.00
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.
CMV control lentivirus (Zeo)
CMV-Null-Zeo 1 x107 IFU/ml x 200ul
EUR 349.00
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the Zeocin selection under RSV promoter.
GFP Expressing Human Glioblastoma Cells
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GFP Expressing Human Prostate Carcinoma Cells (DU 145)
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Lenti-HOXA10-GFP Lentivirus
LV646 10 ml
EUR 811.00
Lenti-HOXB8-GFP Lentivirus
LV634 10 ml
EUR 811.00
Lenti-HOXA9-GFP Lentivirus
LV640 10 ml
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ISRE-Luc (GFP) Lentivirus 
LVP939-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-Rluc (GFP) Lentivirus 
LVP940-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Bsd) Lentivirus 
LVP941-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Neo) Lentivirus 
LVP941-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (Puro) Lentivirus 
LVP941-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-GFP (RFP) Lentivirus 
LVP941-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-RFP (GFP) Lentivirus 
LVP942-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Luc (GFP) Lentivirus 
LVP943-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Rluc (GFP) Lentivirus 
LVP944-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Bsd) Lentivirus 
LVP945-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Neo) Lentivirus 
LVP945-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (Puro) Lentivirus 
LVP945-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-GFP (RFP) Lentivirus 
LVP945-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-RFP (GFP) Lentivirus 
LVP946-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Luc (GFP) Lentivirus 
LVP947-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Rluc (GFP) Lentivirus 
LVP948-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Bsd) Lentivirus 
LVP949-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Neo) Lentivirus 
LVP949-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (Puro) Lentivirus 
LVP949-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-GFP (RFP) Lentivirus 
LVP949-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-RFP (GFP) Lentivirus 
LVP950-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Luc (GFP) Lentivirus 
LVP951-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Rluc (GFP) Lentivirus 
LVP952-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Bsd) Lentivirus 
LVP953-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Neo) Lentivirus 
LVP953-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (Puro) Lentivirus 
LVP953-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-GFP (RFP) Lentivirus 
LVP953-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-RFP (GFP) Lentivirus 
LVP954-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Luc (GFP) Lentivirus 
LVP955-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Rluc (GFP) Lentivirus 
LVP956-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Bsd) Lentivirus 
LVP957-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Neo) Lentivirus 
LVP957-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (Puro) Lentivirus 
LVP957-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-GFP (RFP) Lentivirus 
LVP957-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-RFP (GFP) Lentivirus 
LVP958-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Luc (GFP) Lentivirus 
LVP959-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Rluc (GFP) Lentivirus 
LVP960-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Bsd) Lentivirus 
LVP961-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Neo) Lentivirus 
LVP961-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (Puro) Lentivirus 
LVP961-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-GFP (RFP) Lentivirus 
LVP961-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-RFP (GFP) Lentivirus 
LVP962-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Luc (GFP) Lentivirus 
LVP963-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Rluc (GFP) Lentivirus 
LVP964-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Bsd) Lentivirus 
LVP965-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Neo) Lentivirus 
LVP965-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (Puro) Lentivirus 
LVP965-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-GFP (RFP) Lentivirus 
LVP965-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-RFP (GFP) Lentivirus 
LVP966-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-Luc (GFP) Lentivirus 
LVP967-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-Rluc (GFP) Lentivirus 
LVP968-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Bsd) Lentivirus 
LVP969-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Neo) Lentivirus 
LVP969-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (Puro) Lentivirus 
LVP969-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-GFP (RFP) Lentivirus 
LVP969-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-RFP (GFP) Lentivirus 
LVP970-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Luc (GFP) Lentivirus 
LVP971-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Rluc (GFP) Lentivirus 
LVP972-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Bsd) Lentivirus 
LVP973-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Neo) Lentivirus 
LVP973-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (Puro) Lentivirus 
LVP973-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-GFP (RFP) Lentivirus 
LVP973-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-RFP (GFP) Lentivirus 
LVP974-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Luc (GFP) Lentivirus 
LVP975-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Rluc (GFP) Lentivirus 
LVP976-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Bsd) Lentivirus 
LVP977-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Neo) Lentivirus 
LVP977-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (Puro) Lentivirus 
LVP977-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-GFP (RFP) Lentivirus 
LVP977-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-RFP (GFP) Lentivirus 
LVP978-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Luc (GFP) Lentivirus 
LVP979-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Rluc (GFP) Lentivirus 
LVP980-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Bsd) Lentivirus 
LVP981-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Neo) Lentivirus 
LVP981-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (Puro) Lentivirus
LVP981-P 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-GFP (RFP) Lentivirus 
LVP981-R 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-RFP (GFP) Lentivirus 
LVP982-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Luc (GFP) Lentivirus 
LVP983-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Rluc (GFP) Lentivirus 
LVP984-G 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-GFP (Bsd) Lentivirus 
LVP985-B 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-GFP (Neo) Lentivirus 
LVP985-N 1x107 IFU/ml x 200ul
EUR 552.00
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

Establishment of a physiologically relevant human hepatocyte-like cell system for in vitro translational research has been hampered by the limited availability of cell models that accurately reflect human biology and the pathophysiology of human disease. Here we report a robust, reproducible, and scalable protocol for the generation of hepatic organoids from human induced pluripotent stem cells (hiPSCs) using short exposure to nonengineered matrices.

 These hepatic organoids follow defined stages of hepatic development and express higher levels of early (hepatocyte nuclear factor 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this model to explore the biology of the pleiotropic TRIB1 (Tribbles-1) gene associated with a number of metabolic traits, including nonalcoholic fatty liver disease and plasma lipids.

We used genome editing to delete the TRIB1 gene in hiPSCs and compared TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs under both 2D culture and three-dimensional (3D) organoid conditions. Under conventional 2D culture conditions, TRIB1-deficient HLCs showed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In contrast, when cultured as 3D hepatic organoids, the differentiation defects were rescued, and a clear lipid-related phenotype was noted in the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work supports the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, including the functional interrogation of genes identified through human genetic investigation.

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price
Description: The best epigenetics products

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 243.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Membrane Protein from Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 285.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 214.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194.00
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 207.00

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 213.00

293/GFP Cell Line

AKR-200 1 vial
EUR 572.00
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572.00
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572.00
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572.00
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572.00
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 164.00

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 128.00

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 467.00
  • Category: Exosomes

MCF-10A

C0006015 One Frozen vial
EUR 467.00

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 213.00

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1354.00

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1354.00

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1354.00

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354.00

293AD Cell Line

AD-100 1 vial
EUR 461.00
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508.00
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508.00
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508.00
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572.00

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572.00

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572.00

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2333.00

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2333.00

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2333.00

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2333.00

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2333.00

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2333.00

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2333.00

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2333.00

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1394.00

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1394.00

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1394.00

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1394.00

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1394.00

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2333.00

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2333.00

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2333.00

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2333.00

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2333.00

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2333.00

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572.00
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572.00
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920.00
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920.00
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920.00
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 712.00

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345.00
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167.00

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Human macrophage chemotatic factor,MCF ELISA Kit

201-12-0070 96 tests
EUR 440.00
  • This macrophage chemotatic factor ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human macrophage chemotatic factor,MCF ELISA Kit

CN-04498H1 96T
EUR 452.00

Human macrophage chemotatic factor,MCF ELISA Kit

CN-04498H2 48T
EUR 302.00

Monkey Macrophage chemotactic Factor (MCF) ELISA Kit

abx359979-96tests 96 tests
EUR 825.00
  • Shipped within 5-12 working days.

Pig Macrophage chemotactic Factor (MCF) ELISA Kit

abx361774-96tests 96 tests
EUR 825.00
  • Shipped within 5-12 working days.

Rabbit Macrophage chemotactic Factor (MCF) ELISA Kit

abx362676-96tests 96 tests
EUR 825.00
  • Shipped within 5-12 working days.

Human Macrophage Chemotactic Factor (MCF) ELISA Kit

abx354411-96tests 96 tests
EUR 786.00
  • Shipped within 5-12 working days.

Chicken Macrophage chemotactic Factor (MCF) ELISA Kit

abx356572-96tests 96 tests
EUR 825.00
  • Shipped within 5-12 working days.

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-48T 48T
EUR 402.00

Canine macrophage chemotatic factor(MCF)ELISA Kit

GA-E0087CN-96T 96T
EUR 684.00

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-48T 48T
EUR 289.00

Human macrophage chemotatic factor(MCF)ELISA Kit

GA-E0109HM-96T 96T
EUR 466.00

Human macrophage chemotatic factor(MCF)ELISA Kit

QY-E02720 96T
EUR 361.00

GFP Recombinant Adenovirus

ADV-004 50 ?L
EUR 891.00
Description: Premade recombinant adenovirus containing the GFP gene

GFP Quantitation Kit

AKR-120 100 assays
EUR 577.00
Description: Cell Biolabs? GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.

GFP ELISA Kit

AKR-121 96 assays
EUR 734.00
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP ELISA Kit

AKR-121-5 5 x 96 assays
EUR 3025.00
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP Lentivirus Control

LTV-300 1 vial
EUR 566.00
Description: HIV-1 Lentivirus

Recombinant Human Nuclear Import 7 Homolog

7-05707 5µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05708 25µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05709 1mg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05800 5µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05801 20µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05802 1mg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07543 100µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07544 500µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07545 1000µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00184 2µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00185 10µg Ask for price

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