Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT

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fmblab

Propofol Affects Non-Small-Cell Lung Cancer Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

Background: Propofol is a common sedative-hypnotic drug traditionally used for inducing and maintaining general anesthesia. Recent studies have drawn attention to the nonanesthetic effects of propofol, but the potential mechanism by which propofol suppresses non-small-cell lung cancer (NSCLC) progression has not been fully elucidated.

Methods: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to treat A549 cells for 1, 4, and 12 hours and Cell Counting Kit-8 (CCK-8) to detect proliferation. Apoptosis was measured with flow cytometry. We also transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or negative control ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 small interfering ribonucleic acid (siRNA) or negative control. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression were detected using Western blotting, whereas miR-21 expression was examined by real-time polymerase chain reaction (RT-PCR). In vivo, nude mice were given injections of A549 cells to grow xenograft tumors; 8 days later, the mice were intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors were then collected from mice and analyzed by immunohistochemistry and Western blotting.

Results: Propofol inhibited growth (1 hour, P = .001; 4 hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; 4 hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent manner. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive effects of propofol on A549 cells by decreasing PTEN protein

expression (mean differences [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), resulting in an increase in pAKT levels (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol exposure. In vivo, propofol treatment reduced NSCLC tumor growth (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

Conclusions: Our study indicated that propofol inhibited A549 cell growth, accelerated apoptosis via the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell growth, and promoted apoptosis in vivo. Our findings provide new implications for propofol in cancer therapy and indicate that propofol is extremely advantageous in surgical treatment.

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EF1a control lentivirus (Hygro)
EF1a-Null-Hygro 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter.
Negative Control Luciferase Lentivirus
79578 500 µl x 2
EUR 860
Description: The Negative Control Lentivirus (Firefly Luciferase) are replication incompetent, HIV-based, VSV-G pseudo typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.
Negative Control eGFP Reporter Lentivirus
79927 500 µl x 2
EUR 835
Description: The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.
Expression Negative Control Lentivirus (G418)
79902-G 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
Expression Negative Control Lentivirus (Puromycin)
79902-P 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
Expression Negative Control Lentivirus (Hygromycin)
79902-H 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
GFP inducible control lentiviral particles 
LVP024 1x10e7 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral particles expressing codon optimized GFP under tetracycline inducible suCMV promoter. Particles contain a blasticidin-RFP fusion dual marker under the constitutive RSV promoter.
CMV Control lentiviral particles (GFP-Bsd)
CMV-Null-GB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Blasticidin fusion marker under RSV promoter.
CMV Control lentiviral particles (GFP-Puro)
CMV-Null-GP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter.
EF1a Control lentiviral particles (GFP-Bsd)
EF1a-Null-GB 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Blasticidin fusion marker under RSV promoter.
EF1a Control lentiviral particles (GFP-Puro)
EF1a-Null-GP 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter.
GFP-RFP fusion control lentiviral particles
LVP442 1x10e7 IFU/ml x 200ul
EUR 541.2
Description: Pre-made lentiviral particles expressing GFP-RFP fusion contruct under suCMV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
GFP-Null fusion control lentiviral particles
Null-G-(Puro) 1x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made GFP-Null fusion Control lentivirus containing puromycin marker. GFP fusioned with a non-targeting Null sequence for evenly fluorescent signal distribution. Virus contains a Puromycin selection marker.
pLenti-GFP Lentiviral Control Vector
LTV-400 100 µL
EUR 741.6
Description: Use this control vector to co-transfect along with lentivirus packaging vectors to make a recombinant control lentivirus.
GFP inducible control lentiviral particles in PBS
LVP024-PBS 5x107 IFU/ml x 200ul
EUR 852
Description: Pre-made lentiviral particles expressing codon optimized GFP under tetracycline inducible suCMV promoter. Particles contain a blasticidin-RFP fusion dual marker under the constitutive RSV promoter, provided in PBS solution
CMV Control lentiviral particles (GFP-Bsd) in PBS
CMV-Null-GB-PBS 1 x108 IFU/ml x 200ul
EUR 852
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Blasticidin fusion marker under RSV promoter. The virus was concentrated and provided in PBS solution.
CMV Control lentiviral particles (GFP-Puro) in PBS
CMV-Null-GP-PBS 1 x108 IFU/ml x 200ul
EUR 852
Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter. The virus was concentrated and provided in PBS solution.
EF1a Control lentiviral particles (GFP-Bsd) in PBS
EF1a-Null-GB-PBS 1 x108 IFU/ml x 200ul
EUR 852
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Blasticidin fusion marker under RSV promoter. The virus was concentrated and provided in PBS solution.
EF1a Control lentiviral particles (GFP-Puro) in PBS
EF1a-Null-GP-PBS 1 x108 IFU/ml x 200ul
EUR 852
Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It also has the GFP-Puromycin fusion marker under RSV promoter. The virus was concentrated and provided in PBS solution.
pSMPUW-GFP-Puro Lentiviral Control Vector
LTV-401 10 µg
EUR 741.6
Description: Use this control vector to co-transfect along with lentivirus packaging vectors to make a recombinant control lentivirus.
AR-RFP (GFP) Lentivirus 
LVP913-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-Luc (GFP) Lentivirus 
LVP914-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-RFP (GFP) Lentivirus 
LVP950-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Luc (GFP) Lentivirus 
LVP951-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-RFP (GFP) Lentivirus 
LVP970-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Luc (GFP) Lentivirus 
LVP971-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-RFP (GFP) Lentivirus 
LVP974-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Luc (GFP) Lentivirus 
LVP975-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
MB-RFP (GFP) Lentivirus 
LVP1022-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
MB-Luc (GFP) Lentivirus 
LVP1023-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AR-Rluc (GFP) Lentivirus 
LVP915-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-RFP (GFP) Lentivirus 
LVP946-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Luc (GFP) Lentivirus 
LVP947-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
G_RE-Rluc (GFP) Lentivirus 
LVP952-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-RFP (GFP) Lentivirus 
LVP954-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Luc (GFP) Lentivirus 
LVP955-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-RFP (GFP) Lentivirus 
LVP958-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Luc (GFP) Lentivirus 
LVP959-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-RFP (GFP) Lentivirus 
LVP962-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Luc (GFP) Lentivirus 
LVP963-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
H_RE-Rluc (GFP) Lentivirus 
LVP972-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ER-Rluc (GFP) Lentivirus 
LVP976-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-RFP (GFP) Lentivirus 
LVP978-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Luc (GFP) Lentivirus 
LVP979-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-RFP (GFP) Lentivirus 
LVP982-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Luc (GFP) Lentivirus 
LVP983-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
B29-RFP (GFP) Lentivirus 
LVP994-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
B29-Luc (GFP) Lentivirus 
LVP995-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
MB-Rluc (GFP) Lentivirus 
LVP1024-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SPB-RFP (GFP) Lentivirus 
LVP1026-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SPB-Luc (GFP) Lentivirus 
LVP1027-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
aFP-RFP (GFP) Lentivirus 
LVP1034-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
aFP-Luc (GFP) Lentivirus 
LVP1035-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
HE4-RFP (GFP) Lentivirus 
LVP1042-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
HE4-Luc (GFP) Lentivirus 
LVP1043-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
PSA-RFP (GFP) Lentivirus 
LVP1046-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
PSA-Luc (GFP) Lentivirus 
LVP1047-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-RFP (GFP) Lentivirus 
LVP938-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ISRE-Luc (GFP) Lentivirus 
LVP939-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-RFP (GFP) Lentivirus 
LVP942-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CRE_TRE-Luc (GFP) Lentivirus 
LVP943-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Gli-Rluc (GFP) Lentivirus 
LVP948-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
AP1-Rluc (GFP) Lentivirus 
LVP956-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SRE-Rluc (GFP) Lentivirus 
LVP960-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
RBP_JK-Rluc (GFP) Lentivirus 
LVP964-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-RFP (GFP) Lentivirus 
LVP966-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
NFkB-Luc (GFP) Lentivirus 
LVP967-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
P53-Rluc (GFP) Lentivirus 
LVP980-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
ARE-Rluc (GFP) Lentivirus 
LVP984-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-RFP (GFP) Lentivirus 
LVP986-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CEBP-Luc (GFP) Lentivirus 
LVP987-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
B29-Rluc (GFP) Lentivirus 
LVP996-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD14-RFP (GFP) Lentivirus 
LVP998-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD14-Luc (GFP) Lentivirus 
LVP999-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD43-RFP (GFP) Lentivirus 
LVP1002-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD43-Luc (GFP) Lentivirus 
LVP1003-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD45-RFP (GFP) Lentivirus 
LVP1006-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD45-Luc (GFP) Lentivirus 
LVP1007-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD68-RFP (GFP) Lentivirus 
LVP1010-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
CD68-Luc (GFP) Lentivirus 
LVP1011-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Flt1-RFP (GFP) Lentivirus 
LVP1014-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
Flt1-Luc (GFP) Lentivirus 
LVP1015-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SPB-Rluc (GFP) Lentivirus 
LVP1028-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SYN1-RFP (GFP) Lentivirus 
LVP1030-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
SYN1-Luc (GFP) Lentivirus 
LVP1031-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
aFP-Rluc (GFP) Lentivirus 
LVP1036-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
HE4-Rluc (GFP) Lentivirus 
LVP1044-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
PSA-Rluc (GFP) Lentivirus 
LVP1048-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-RFP (GFP) Lentivirus 
LVP1120-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.
EGR1-RFP (GFP) Lentivirus 
LVP1120-G-PBS 1x108 IFU/ml x 200ul
EUR 852
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in PBS solution.

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

Establishment of a physiologically relevant human hepatocyte-like cell system for in vitro translational research has been hampered by the limited availability of cell models that accurately reflect human biology and the pathophysiology of human disease. Here we report a robust, reproducible, and scalable protocol for the generation of hepatic organoids from human induced pluripotent stem cells (hiPSCs) using short exposure to nonengineered matrices.

 These hepatic organoids follow defined stages of hepatic development and express higher levels of early (hepatocyte nuclear factor 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this model to explore the biology of the pleiotropic TRIB1 (Tribbles-1) gene associated with a number of metabolic traits, including nonalcoholic fatty liver disease and plasma lipids.

We used genome editing to delete the TRIB1 gene in hiPSCs and compared TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs under both 2D culture and three-dimensional (3D) organoid conditions. Under conventional 2D culture conditions, TRIB1-deficient HLCs showed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In contrast, when cultured as 3D hepatic organoids, the differentiation defects were rescued, and a clear lipid-related phenotype was noted in the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work supports the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, including the functional interrogation of genes identified through human genetic investigation.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

cDNA - Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 385

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

Total RNA - Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 191

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

Genomic DNA - Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 245

Total Protein - Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 188

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

Membrane Protein - Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 270

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 238

MCF-7 cells

C0006008 One Frozen vial
EUR 546

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Human Mcf-7 Whole Cell Lysate

LYSATE0024 200ug
EUR 180
Description: This cell lysate is prepared from human mcf-7 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

MCF-7-LUC cells

S0006002 One Frozen vial
EUR 651.6

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 409

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 255.6

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 196.8

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 153.6

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

MCF 7 Membrane Lysate

XBL-10442 0.1 mg
EUR 619.8
Description: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size

CBA-135-T 24 assays
EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

GFP ELISA Kit

AKR-121 96 assays
EUR 880.8
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP ELISA Kit

AKR-121-5 5 x 96 assays
EUR 3630
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

GFP Quantitation Kit

AKR-120 100 assays
EUR 692.4
Description: Cell Biolabs? GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.

GFP Lentivirus Control

LTV-300 1 vial
EUR 679.2
Description: HIV-1 Lentivirus

GFP Recombinant Adenovirus

ADV-004 50 ?L
EUR 1069.2
Description: Premade recombinant adenovirus containing the GFP gene

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size

CBA-140-T 24 assays
EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

MCF7 / GFP (Puromycin) Stable Cell Line

SC050-G 2 x 106 cell/ml x 1ml
EUR 1452
Description: GFP expression stable cell line in MCF7 cells with Puromycin resistance

Collagen-based Cell Contraction Assay

CBA-201 24 assays
EUR 582
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 490.8
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Radius 24-Well Cell Migration Assay

CBA-125 24 assays
EUR 602.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay

CBA-125-5 5 x 24 assays
EUR 2362.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126 96 assays
EUR 686.4
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 5 x 96 assays
EUR 2697.6
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127 384 assays
EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay

CBA-127-5 5 x 384 wells
EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037 48 assays
EUR 622.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 48-Well Cell Migration Assay

CBA-5037-5 5 x 48 assays
EUR 2454
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

MCF-7 Nuclear Extract (H2O2)

1642-100 each
EUR 248.4

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 637.2
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect 96-well Cell Transformation Assay

CBA-130 96 assays
EUR 866.4
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 5 x 96 assays
EUR 3463.2
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 24-well Cell Invasion, Fluorometric

CBA-111 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

CytoSelect 96-well Cell Invasion, Fluorometric

CBA-112 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells.

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240 96 assays
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

GFP ELISA Kit, Trial Size

AKR-121-T 32 assays
EUR 470.4
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

CytoSelect 24-Well Cell Co-Culture System

CBA-160 24 assays
EUR 525.6
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

CytoSelect 24-Well Cell Co-Culture System

CBA-160-5 5 x 24 assays
EUR 2078.4
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions.

Mcf2 3'UTR GFP Stable Cell Line

TU162989 1.0 ml Ask for price

MCF2 3'UTR GFP Stable Cell Line

TU063111 1.0 ml
EUR 1672.8

Mcf2 3'UTR GFP Stable Cell Line

TU262955 1.0 ml Ask for price

CytoSelect 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 762
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)

CBA-250 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)

CBA-253 10 mL
EUR 490.8
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

GFP-Rac1 Expression Vector Set

STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set

STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-Cdc42 Expression Vector Set

STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

MCF2L 3'UTR GFP Stable Cell Line

TU063112 1.0 ml
EUR 2799.6

Mcf2l 3'UTR GFP Stable Cell Line

TU262956 1.0 ml Ask for price

CytoSelect 24-well Cell Invasion Assay, Colorimetric

CBA-110 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells.

MCF2L2 3'UTR GFP Stable Cell Line

TU063113 1.0 ml
EUR 1825.2

CytoSelect 24-well Laminin Cell Invasion, Colorimetric

CBA-110-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 24-well Collagen Cell Invasion, Colorimetric

CBA-110-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

Radius 24-Well Cell Migration Assay, (Laminin Coated)

CBA-125-LN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145 384 assays
EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric

CBA-145-5 5 x 384 assays
EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

StemTAG PCR Primer Set for Stem Cell Characterization

CBA-303 1 kit
EUR 470.4
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 96 assays
EUR 762
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)

CBA-155 5 preps
EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Radius 24-Well Cell Migration Assay, (Fibronectin Coated)

CBA-125-FN 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 24-Well Cell Migration Assay, (ECM Array Coated)

CBA-125-ECM 24 wells
EUR 838.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit

CBA-254 96 assays
EUR 672
Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts.  The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. 

CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric)

CBA-056 48 assays
EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)

CBA-057 48 assays
EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric

CBA-111-LN 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

Radius 24-Well Cell Migration Assay, (Collagen I Coated)

CBA-125-COL 24 assays
EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 24-well Collagen Cell Invasion Assay, Fluorometric

CBA-111-COL 12 assays
EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL 96 assays
EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

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