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fmblab

Kiss1R identification and biodistribution analysis utilizing a western ligand blot

Kiss1R identification and biodistribution analysis utilizing a western ligand blot and ligand-derivative stain with a FITC-kisspeptin by-product   It isn’t always easy to determine explicit antibodies in opposition to receptors. Most receptors are hydrophobic and have tough three-dimensional constructions, making them powerful to utilize as immunogens. Thus, we developed receptor detection methods with a fluorescein-labeled ligand as an antibody […]

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fmblab

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fmblab

Enchancment of immunodetection of the transcription issue C/EBP homologous protein

Enchancment of immunodetection of the transcription issue C/EBP homologous protein by western blot. Accumulation of misfolded proteins throughout the endoplasmic reticulum (ER) induces an unfolded protein response (UPR) that both restores homeostasis or triggers apoptosis in case of adaptation failure. The three activated branches of UPR result in IRE1-, PERK- and ATF6– dependent transcriptional induction of the […]